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Ch.16 Amino Acids, Proteins, and Enzymes
Timberlake - Chemistry: An Introduction to General, Organic, and Biological Chemistry 13th Edition
Timberlake13th EditionChemistry: An Introduction to General, Organic, and Biological ChemistryISBN: 9780134421353Not the one you use?Change textbook
Chapter 16, Problem 45

The following graph shows the activity versus pH curves for pepsin, sucrase, and trypsin. Estimate the optimum pH for each.
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1
Examine the graph provided in the problem. Identify the curves corresponding to pepsin, sucrase, and trypsin. Each curve represents the activity of the enzyme at different pH levels.
Locate the peak of each curve on the graph. The peak represents the point of maximum activity for the enzyme, which corresponds to its optimum pH.
For pepsin, observe the pH value at which its activity is highest. This is the optimum pH for pepsin.
For sucrase, identify the pH value at the peak of its activity curve. This is the optimum pH for sucrase.
For trypsin, determine the pH value at the highest point of its activity curve. This is the optimum pH for trypsin.

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Key Concepts

Here are the essential concepts you must grasp in order to answer the question correctly.

Enzyme Activity

Enzyme activity refers to the rate at which an enzyme catalyzes a reaction. It is influenced by various factors, including substrate concentration, temperature, and pH. Understanding how these factors affect enzyme activity is crucial for determining the optimum conditions under which enzymes like pepsin, sucrase, and trypsin function most effectively.
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pH and Enzyme Function

pH is a measure of the acidity or alkalinity of a solution, which can significantly impact enzyme structure and function. Each enzyme has an optimum pH range where it performs best; deviations from this range can lead to decreased activity or denaturation. For example, pepsin operates optimally in acidic conditions, while trypsin functions best in a more neutral to slightly alkaline environment.
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Optimum pH

Optimum pH is the specific pH level at which an enzyme exhibits maximum activity. This value varies among different enzymes due to their unique structures and the environments in which they typically operate. Estimating the optimum pH for enzymes like pepsin, sucrase, and trypsin involves analyzing their activity curves in relation to pH, allowing for a better understanding of their functional conditions.
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