Which recombinant DNA technique is used to replicate copies of a DNA molecule?


a. PCR
b. Gel electrophoresis
c. Electroporation
d. Reverse transcription

Verified step by step guidance

1. Understand the goal: The question asks which recombinant DNA technique is used to replicate copies of a DNA molecule, meaning it focuses on amplifying or making many copies of a specific DNA segment.
2. Review each option: - PCR (Polymerase Chain Reaction) is a method that amplifies DNA by repeatedly copying a target DNA sequence. - Gel electrophoresis is a technique used to separate DNA fragments based on size, not to replicate DNA. - Electroporation is a method to introduce DNA into cells by applying an electric field, not for DNA replication. - Reverse transcription is the process of converting RNA into complementary DNA (cDNA), not directly replicating DNA.
3. Identify the key function: Since the question is about replicating or amplifying DNA copies, focus on the technique that specifically performs DNA amplification.
4. Recall the principle of PCR: PCR uses cycles of heating and cooling along with DNA polymerase enzyme to exponentially amplify a specific DNA sequence, producing millions of copies from a small initial amount.
5. Conclude that the correct recombinant DNA technique for replicating copies of a DNA molecule is PCR.

Key Concepts

Polymerase Chain Reaction (PCR)

PCR is a technique used to amplify specific DNA sequences, creating millions of copies from a small initial sample. It involves repeated cycles of denaturation, annealing of primers, and extension by DNA polymerase, enabling rapid and precise DNA replication in vitro.

Gel Electrophoresis

Gel electrophoresis is a method used to separate DNA fragments based on size by applying an electric field to a gel matrix. It does not replicate DNA but helps analyze and visualize DNA fragments after amplification or digestion.

Electroporation

Electroporation is a technique that uses electrical pulses to create temporary pores in cell membranes, allowing DNA or other molecules to enter cells. It facilitates DNA uptake but does not itself replicate DNA molecules.