In general, when recombination experiments are conducted with bac...

Question

In general, when recombination experiments are conducted with bacteria, participating bacteria are mixed in complete medium, then transferred to a minimal growth medium. Why isn't the protocol reversed: minimal medium first, complete medium second?

Accepted Answer

Everyone. Let's take a look at this question together, chemically defined media are blank media that precisely meet and organisms nutritional needs, whereas blank media are rich cultures that provide microorganisms access to a wide variety of nutrients and other growth stimuli. So if we're talking about a chemically defined media that precisely meats and organisms nutritional needs, meaning that it only contains what an organism needs. We could say that it contains the minimum requirements or that wild type growth. And then on the other hand, a media containing rich cultures that provide those microorganisms with access to a wide variety of nutrients and other growth stimuli. We could say that that media is complex with the base needs as well as additional resources that those microorganisms might want in addition to the minimum requirements. So that would make answer choice B the correct answer. Because when we're talking about that chemically defined media that precisely meet the organisms nutritional needs, were saying that it has the minimum requirements for that wild type growth and then a culture that is rich in nutrients as well as some extra or additional growth stimuli. Were saying that it is complex, meaning it has those base needs or those minimum requirements and the additional resources. So answer choice B is the correct answer. I hope you found this video to be helpful. Thank you and goodbye

In general, when recombination experiments are conducted with bacteria, participating bacteria are mixed in complete medium, then transferred to a minimal growth medium. Why isn't the protocol reversed: minimal medium first, complete medium second?

Watch next