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Multiple Choice
In a DNA strand, how can you identify the 5' and 3' ends based on the chemical structure of the sugar-phosphate backbone?
A
The 5' end has a phosphate group attached to the 5' carbon of deoxyribose, while the 3' end has a free hydroxyl (3'-OH) group on the 3' carbon.
B
The 5' end is identified by the presence of a free nitrogenous base, while the 3' end is identified by the presence of hydrogen bonds to the complementary strand.
C
The 5' end always corresponds to the end of the strand with A–T base pairs, while the 3' end corresponds to the end with G–C base pairs.
D
The 5' end has a free hydroxyl (5'-OH) group on the 5' carbon, while the 3' end has a phosphate group attached to the 3' carbon.