Question

Most of the techniques described in this chapter (blotting, cloning, PCR, etc.) are dependent on hybridization (annealing) between different populations of nucleic acids. Length of the strands, temperature, and percentage of GC nucleotides weigh considerably on hybridization. Two other components commonly used in hybridization protocols are monovalent ions and formamide. A formula that takes monovalent Na⁺ ions ((M[Na⁺]) and formamide concentrations into consideration to compute a Tₘ (temperature of melting) is as follows:

Tₘ=81.5+16.6(log M[Na+])+0.41(%GC)−0.72(%formamide)

Given that formamide competes for hydrogen bond locations on nucleic acid bases and monovalent cations are attracted to the negative charges on nucleic acids, explain why the Tₘ varies as described in part (a).

Accepted Answer

Hello everyone here we have a question asking which of the following statements is true regarding the kneeling temperature in pcr, primers, Nucleotide makeup, most notably the proportion of timing basis affects its melting temperature. They are directly proportional, meaning the melting temperature of a primer rises. As the proportion of timing basis increases. A primer with higher melting temperature needs a higher and kneeling temperature for effective and healing to the template D. N. A. And this is because the enabling temperature is normally a few degrees below the melting temperature of a primer. So a high percent of the, I mean in a primer causes the enabling temperature to pcr to rise. So our answer here is B. The kneeling temperature increases when a primer has high percent. T. Thank you for watching. Bye.

Watch next