Question

There are a variety of circumstances under which rapid results using multiple markers in PCR amplifications are highly desired, such as in forensics, pathogen analysis, or detection of genetically modified organisms. In multiplex PCR, multiple sets of primers are used, often with less success than when applied to PCR as individual sets. Numerous studies have been conducted to optimize procedures, but each has described the process as time consuming and often unsuccessful. Considering the information given in Problem 30, why should multiplex PCR be any different than single primer set PCR in terms of dependability and ease of optimization?

Accepted Answer

Everyone. Let's take a look at this question together which of the following terms is used to describe the phenomenon of non specific amplification in multiplex PCR. So let's recall what we know about non specific amplification and multiplex PCR to figure out which of the following terms is used to describe it. So we know that non specific amplification can be caused by amplified products that come from one sample are transferred to another sample. And so the term that we use to describe the phenomenon of these amplified products from one sample being transferred to another is answer choice a cross contamination because in that multiplex PCR cross contamination can happen when we take those amplified products from one sample and transfer it to another. So answer choice A is the correct answer. I hope you found this video to be helpful. Thank you and goodbye.

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