A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted three times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 17 plaques. What is the initial density of bacteriophages in the original 1 mL?
Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
5. Genetics of Bacteria and Viruses
Bacteriophage Genetics
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Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
A mixed infection of two bacteriophage strains is often used for what purpose?
A
To sequence bacteriophage genomes
B
To measure bacteria resistance
C
To study conjugation
D
To map bacteriophage genes
Verified step by step guidance1
Understand that bacteriophages are viruses that infect bacteria, and studying their genetics can provide insights into viral behavior and bacterial interactions.
Recognize that a mixed infection involves two different strains of bacteriophages infecting the same bacterial host, which can lead to recombination events between the phage genomes.
Learn that genetic recombination in bacteriophages can be used to determine the relative positions of genes on the phage genome, a process known as genetic mapping.
Realize that by analyzing the frequency of recombination between different genetic markers, researchers can infer the distance between genes and construct a genetic map.
Conclude that the primary purpose of using a mixed infection of two bacteriophage strains is to map bacteriophage genes, as it allows for the study of genetic linkage and recombination events.
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