Multiple Choice
In methods for analyzing DNA, gel electrophoresis separates (sorts) DNA molecules primarily on the basis of their:
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18. Molecular Genetic Tools
Methods for Analyzing DNA
Multiple Choice
In the DNA isolation process, detergent is used primarily to accomplish which of the following?
A
Precipitate DNA out of solution by reducing its solubility in water
B
Denature proteins by breaking peptide bonds into individual amino acids
C
Cut DNA into smaller fragments at specific recognition sites
D
Disrupt cell and nuclear membranes by dissolving lipids, helping release DNA into solution
Verified step by step guidance1
Understand the role of detergent in the DNA isolation process: detergents are amphipathic molecules that interact with lipid bilayers.
Recognize that cell and nuclear membranes are composed mainly of lipid bilayers, which act as barriers enclosing cellular contents including DNA.
Know that detergents disrupt these lipid bilayers by dissolving the lipids, effectively breaking down the membranes.
This disruption releases the DNA from inside the nucleus and cytoplasm into the solution, making it accessible for further isolation steps.
Therefore, the primary function of detergent in DNA isolation is to disrupt cell and nuclear membranes by dissolving lipids, facilitating the release of DNA.
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