Multiple Choice
In methods for analyzing DNA, what is most commonly used to cut DNA into specific fragments at defined recognition sequences?
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18. Molecular Genetic Tools
Methods for Analyzing DNA
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In a standard DNA extraction protocol, why is detergent (e.g., SDS) added to the sample?
A
To disrupt cell and nuclear membranes by solubilizing lipids and denaturing membrane proteins, releasing DNA into solution
B
To amplify the DNA by serving as a primer for DNA polymerase
C
To precipitate DNA out of solution by reducing its solubility in water
D
To specifically cut DNA at restriction sites to generate fragments for analysis
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