Multiple Choice
Which method rapidly amplifies (copies) a specific DNA sequence in vitro without using bacteria or cloning in cells?
18. Molecular Genetic Tools
Methods for Analyzing DNA
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In gel electrophoresis as a method for analyzing DNA, why is a DNA ladder (molecular weight marker) useful and why is it included on the gel?
A
It provides DNA fragments of known sizes so you can estimate the base-pair lengths of sample bands by comparing migration distances.
B
It ligates DNA fragments together to increase their size so they migrate more slowly and separate better.
C
It prevents DNA degradation during electrophoresis by inhibiting nucleases in the gel matrix.
D
It stains the DNA in the gel so sample bands become visible under UV light without any additional dye.
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