5. Genetics of Bacteria and Viruses
Bacterial Transformation
3:49 minutes
Problem 23
Textbook Question
Textbook QuestionIn Bacillus subtilis, linkage analysis of two mutant genes affecting the synthesis of two amino acids, tryptophan (trp₂⁻), and tyrosine (trp₁⁻), was performed using transformation. Examine the following data and draw all possible conclusions regarding linkage. What is the purpose of Part B of the experiment? [Reference: E. Nester, M. Schafer, and J. Lederberg (1963).] Donor DNA Recipient Cell Transformants No. trp⁺ tyr⁻ 196 A. trp₂⁺ tyr₁⁺ trp₂⁻ tyr₁⁻ trp⁻ tyr⁺ 328 trp⁺ tyr⁺ 367 trp₂⁺ tyr₁⁻ trp⁺ tyr⁻ 190 B. and trp₂⁻ tyr₁⁻ trp⁻ tyr⁺ 256 trp₂⁻ tyr₁⁺ trp⁺ tyr⁺ 2
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