Question

The human insulin gene contains a number of sequences that are removed in the processing of the mRNA transcript. In spite of the fact that bacterial cells cannot excise these sequences from mRNA transcripts, explain how a gene like this can be cloned into a bacterial cell and produce insulin.

Accepted Answer

Hello everyone and welcome to today's video. So which of the following is our vector that contains the F one origin of replication of the F one page and can be replicated in a cell like a plasma. Well, let's go over each of our answer choices so that we may solve this problem. First of all, we have this echo or one will remember that when we see something like this, this are, is going to be standing for restriction enzyme. This is not going to be a vector or something that can be replicated. So we're going to cancel it up. Then we have Y A C and this is actually going to be standing for just artificial chromosome and it can actually enter the cell and be used for DNA replication just like a plasma. However, it is not going to contain this F one origin of replication of the F one of each. So we're going to cancel this out. Then we have this fage mid. Well, this fage mid is a vector that is going to be containing the F one origin of replication of the F one page, which is just what the question is asking about because of this, it can enter the sale and be replicated just like a plasma because of this. We're going to be highlighting answer choice C as a final answer to our question. I really hope this helped you and I hope to see you on the next one.

The human insulin gene contains a number of sequences that are removed in the processing of the mRNA transcript. In spite of the fact that bacterial cells cannot excise these sequences from mRNA transcripts, explain how a gene like this can be cloned into a bacterial cell and produce insulin.

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