Multiple Choice
In a standard DNA extraction protocol, what is the primary purpose of adding isopropyl alcohol to the lysate?
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18. Molecular Genetic Tools
Methods for Analyzing DNA
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DNA fragments can be separated by gel electrophoresis because they differ in which property that affects their migration through the gel under an electric field?
A
Length (size), which causes smaller fragments to migrate faster through the gel matrix
B
Net positive charge differences among DNA fragments at neutral pH
C
Degree of double-helix supercoiling, which is the main determinant of migration for all linear fragments
D
Nucleotide sequence, because gels separate fragments based primarily on base composition
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