Multiple Choice
In agarose gel electrophoresis, how are DNA or RNA molecules typically visualized on the gel after separation?
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18. Molecular Genetic Tools
Methods for Analyzing DNA
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In which direction does DNA move during agarose gel electrophoresis, and why?
A
Toward the positive electrode (anode) because DNA has a net negative charge due to its phosphate backbone.
B
It moves randomly through the gel because the electric field affects only the loading dye, not DNA.
C
It remains stationary in the wells because DNA is electrically neutral at the pH used for electrophoresis.
D
Toward the negative electrode (cathode) because DNA has a net positive charge due to its phosphate backbone.
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