Which of the following enzymes is responsible for synthesizing new DNA strands during DNA replication in eukaryotic cells?

• A. Helicase
• B. DNA polymerase
• C. Ligase
• D. RNA polymerase

Answer: (B)

Verified step by step guidance

1. Understand the role of each enzyme listed in the context of DNA replication in eukaryotic cells.
2. Recall that Helicase is responsible for unwinding the DNA double helix to create single-stranded templates for replication.
3. Recognize that DNA polymerase is the enzyme that synthesizes new DNA strands by adding nucleotides complementary to the template strand.
4. Note that Ligase functions to join Okazaki fragments on the lagging strand by forming phosphodiester bonds, but does not synthesize new DNA strands.
5. Remember that RNA polymerase synthesizes RNA molecules and is not involved in DNA strand synthesis during replication.