Multiple Choice
In which direction does DNA move during agarose gel electrophoresis, and why?
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18. Molecular Genetic Tools
Methods for Analyzing DNA
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In agarose gel electrophoresis, in which direction does DNA migrate and why?
A
Toward the negative electrode (cathode) because DNA is positively charged at neutral pH.
B
Toward whichever electrode is closest because migration is driven primarily by diffusion, not charge.
C
DNA does not migrate in an electric field; it remains in the wells unless heated.
D
Toward the positive electrode (anode) because the phosphate backbone gives DNA a net negative charge.
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