The two-hybrid method facilitates the discovery of protein–protein interactions. How does this technique work? Can you think of reasons for obtaining a false-positive result, that is, where the proteins encoded by two clones interact in the two-hybrid system but do not interact in the organism in which they naturally occur? Can you think of reasons you might obtain a false-negative result, in which the two proteins interact in vivo but fail to interact in the two-hybrid system?
Table of contents
- 1. Introduction to Genetics51m
- 2. Mendel's Laws of Inheritance3h 37m
- 3. Extensions to Mendelian Inheritance2h 41m
- 4. Genetic Mapping and Linkage2h 28m
- 5. Genetics of Bacteria and Viruses1h 21m
- 6. Chromosomal Variation1h 48m
- 7. DNA and Chromosome Structure56m
- 8. DNA Replication1h 10m
- 9. Mitosis and Meiosis1h 34m
- 10. Transcription1h 0m
- 11. Translation58m
- 12. Gene Regulation in Prokaryotes1h 19m
- 13. Gene Regulation in Eukaryotes44m
- 14. Genetic Control of Development44m
- 15. Genomes and Genomics1h 50m
- 16. Transposable Elements47m
- 17. Mutation, Repair, and Recombination1h 6m
- 18. Molecular Genetic Tools19m
- 19. Cancer Genetics29m
- 20. Quantitative Genetics1h 26m
- 21. Population Genetics50m
- 22. Evolutionary Genetics29m
15. Genomes and Genomics
Proteomics
Problem 20a
Textbook Question
An interactive Web site for the Human Proteome Map (HPM) is available at http://www.humanproteomemap.org. Visit this site, and then answer the question.
How many proteins were identified in this project?
Verified step by step guidance1
Understand that the Human Proteome Map (HPM) project aimed to identify and catalog proteins expressed in various human tissues using mass spectrometry and other proteomic techniques.
Visit the official Human Proteome Map website at http:\/\/www.humanproteomemap.org to access the data and resources provided by the project.
Look for sections such as 'About', 'Data', or 'Summary' on the website, where the total number of proteins identified is typically reported.
Identify the reported total number of proteins detected across all tissues and samples analyzed in the project. This number represents the proteins experimentally confirmed by the HPM.
Note down the number of proteins as stated on the website, which answers the question about how many proteins were identified in the Human Proteome Map project.
Verified video answer for a similar problem:This video solution was recommended by our tutors as helpful for the problem above
Video duration:
2mPlay a video:
Was this helpful?
Key Concepts
Here are the essential concepts you must grasp in order to answer the question correctly.
Human Proteome Map (HPM)
The Human Proteome Map is a comprehensive resource that catalogs proteins expressed in various human tissues and organs. It integrates mass spectrometry data to identify and quantify proteins, providing insights into protein expression patterns across the human body.
Recommended video:
Guided course
Proteomics
Proteomics and Protein Identification
Proteomics is the large-scale study of proteins, focusing on their structures and functions. Protein identification typically involves mass spectrometry techniques that detect peptides, which are then matched to known protein sequences to determine which proteins are present in a sample.
Recommended video:
Guided course
Proteomics
Data Interpretation from Online Biological Databases
Interpreting data from biological databases like the HPM requires understanding how to navigate the site, locate summary statistics, and interpret protein counts. This skill is essential for extracting specific information such as the total number of proteins identified in a project.
Recommended video:
Guided course
Bioinformatics
Related Videos
Related Practice
Textbook Question
391
views
