Multiple Choice
Which genetic principle is fundamental to the modern synthesis of genetics and evolution?
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1. Introduction to Genetics
Modern Genetics
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In CRISPR-Cas9 genome editing, how does Cas9 locate the correct site in DNA to cut?
A
Cas9 scans for methylated cytosines and cuts DNA only at CpG islands.
B
Cas9 uses a guide RNA (gRNA) that base-pairs with a complementary DNA sequence adjacent to a PAM motif, directing Cas9 to cut at that location.
C
Cas9 cuts randomly throughout the genome, and the desired edit is selected by screening afterward.
D
Cas9 recognizes specific amino acid sequences in histone tails to choose where to cut DNA.
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