Multiple Choice
In genetic cloning, the primary function of DNA ligase in recombinant DNA technology is to:
18. Molecular Genetic Tools
Genetic Cloning
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In standard recombinant DNA-based gene cloning, what is typically the first step in the cloning workflow?
A
Amplify and express the cloned gene to produce large amounts of the protein product.
B
Select transformed cells on antibiotic-containing medium and screen for correct inserts.
C
Isolate the DNA containing the gene of interest (e.g., extract genomic DNA or prepare cDNA from mRNA).
D
Transform competent bacterial cells with the recombinant plasmid.
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