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What safety considerations must be taken before CRISPR-Cas is used to edit human embryos to cure disease?
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18. Molecular Genetic Tools
Genetic Cloning
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Join thousands of students who trust us to help them ace their exams!Watch the first videoMultiple Choice
In molecular gene cloning, what is the primary role of DNA ligase?
A
It cuts DNA at specific recognition sequences to generate compatible ends for cloning.
B
It synthesizes a complementary DNA strand using an RNA template.
C
It separates double-stranded DNA into single strands to allow primer annealing.
D
It covalently joins DNA fragments by forming phosphodiester bonds to seal nicks in the sugar-phosphate backbone (e.g., between a vector and an insert).
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