E. coli cells grown on 15N medium are transferred to 14N medium a...

Question

E. coli cells grown on 15N medium are transferred to 14N medium and allowed to grow for two more generations (two rounds of DNA replication). DNA extracted from these cells is centrifuged. What density distribution of DNA would you expect in this experiment?
a. one high-density and one low-density band
b. one intermediate-density band
c. one high-density and one intermediate-density band
d. one low-density and one intermediate-density band

Accepted Answer

Hello everyone here we have a question telling us. Miss Elson install transferred the bacteria from 15 in medium to in medium. They allowed them to replicate for two generations. The D. N. A. Extracted from these sales is centrifuge the D. N. A. Obtained after the first round of replication is a hybrid of one lighter and one heavier strands. Be dense with both heavier strands. See lighter with both lighter strands or D. Have to density classes. So our first strand, our first D. N. A. Has 2 15 inch strands and then it is being put into a medium with 14 in. So once it's put in the 14 in medium it will keep 15 inch strand and it will take a 14 in from the medium. So it will be 15 in and 14 in. So it will be a hybrid of one lighter and one heavier strand. Thank you for watching. Bye.

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