Explain how you might engineer E. coli to produce human growth ho...

Question

Explain how you might engineer E. coli to produce human growth hormone (HGH) using the following: E. coli containing a plasmid, DNA carrying the gene for HGH, DNA ligase, a restriction enzyme, equipment for manipulating and growing bacteria, a method for extracting and purifying the hormone, and an appropriate DNA probe. (Assume that the human HGH gene lacks introns.)

Accepted Answer

Hello everyone and welcome to today's video. So today we're going to be talking about the steps of recumbent DNA technology. So let's begin. Well in case you forgot becoming in DNA technology is this technique that allows us to make genetic manipulations to an isolated genes or gene outside of an organism. And then this gene is going to be introduced inside of the same or another organism so that we can study the effects that the manipulations have on the gene. So the first step that we need to undergo in order to produce this is that we need to isolate this insulin gene or any gene that we're working with. In terms of the recombinant DNA technology, the isolation of the gene is always the first step. Then we need to cut up the plasma DNA at specific locations we make these cuts so that we may then introduce the gene into the plasmid that is going to act as a vector. Then we're going to be joining the insulin gene into the plasmid. This is going to be the next step since we made the cuts of the plasmids. This plasma is going to have a space where we can introduce the insulin gene which is our targeting following. That we're going to be inserting Oh that are coming in DNA plus meat into the bacteria. So now that we have the plasmid with the gene inserted into a acting as our vector. We're going to be inserting it into the bacteria. Which is going to be our target organism. Following that we're going to be selecting and screening the transformed bacteria cells. So after we insert the gene into the bacteria, mia the plasmid, we're going to be studying the effects that this gene is going to have on the bacteria. So this is where we're going to be studying the results. So these are the steps to becoming in DNA technology. And the correct answer choice that describes them is going to be answer choice A is the final answer to our question. And I really hope this video helped you.

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