Table of contents
- 1. Introduction to Genetics(66)
- 2. Mendel's Laws of Inheritance(333)
- 3. Extensions to Mendelian Inheritance(204)
- 4. Genetic Mapping and Linkage(147)
- 5. Genetics of Bacteria and Viruses(101)
- 6. Chromosomal Variation(106)
- 7. DNA and Chromosome Structure(129)
- 8. DNA Replication(69)
- 9. Mitosis and Meiosis(67)
- 10. Transcription(89)
- 11. Translation(150)
- 12. Gene Regulation in Prokaryotes(102)
- 13. Gene Regulation in Eukaryotes(124)
- 14. Genetic Control of Development(76)
- 15. Genomes and Genomics(138)
- 16. Transposable Elements(26)
- 17. Mutation, Repair, and Recombination(148)
- 18. Molecular Genetic Tools(146)
- 19. Cancer Genetics(62)
- 20. Quantitative Genetics(96)
- 21. Population Genetics(112)
- 22. Evolutionary Genetics(38)
5. Genetics of Bacteria and Viruses
Bacterial Transformation
5. Genetics of Bacteria and Viruses
Bacterial Transformation: Videos & Practice Problems
1PRACTICE PROBLEM
The researcher has a bacterial colony containing 500 cells that are a mixture of wild-type and auxotrophic mutants. To identify the auxotrophic mutants, the researcher performs replica plating. The colony is streaked onto a complete medium and allowed to grow. Afterward, a sterile velvet pad is placed onto the bacterial colony, picking up some of the bacterial cells. The pad is then used to make a replica of the bacterial colony onto two different media: minimal media and minimal media supplemented with the nutrient that the auxotrophic mutants require.
What is the purpose of replica plating in identifying auxotrophic mutants in a bacterial colony?
The researcher has a bacterial colony containing 500 cells that are a mixture of wild-type and auxotrophic mutants. To identify the auxotrophic mutants, the researcher performs replica plating. The colony is streaked onto a complete medium and allowed to grow. Afterward, a sterile velvet pad is placed onto the bacterial colony, picking up some of the bacterial cells. The pad is then used to make a replica of the bacterial colony onto two different media: minimal media and minimal media supplemented with the nutrient that the auxotrophic mutants require.
What is the purpose of replica plating in identifying auxotrophic mutants in a bacterial colony?