The researcher has a bacterial colony containing 500 cells that are a mixture of wild-type and auxotrophic mutants. To identify the auxotrophic mutants, the researcher performs replica plating. The colony is streaked onto a complete medium and allowed to grow. Afterward, a sterile velvet pad is placed onto the bacterial colony, picking up some of the bacterial cells. The pad is then used to make a replica of the bacterial colony onto two different media: minimal media and minimal media supplemented with the nutrient that the auxotrophic mutants require. What is the purpose of replica plating in identifying auxotrophic mutants in a bacterial colony?

To allow comparison of the growth of bacterial colonies on different media.

To maximize bacterial growth of all bacteria.

To reduce the number of bacterial colonies.

For better aeration and to promote mutation.

The researcher has a bacterial colony containing 500 cells that a...

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