12. Gene Regulation in Prokaryotes
Lac Operon
12. Gene Regulation in Prokaryotes Lac Operon
9PRACTICE PROBLEM
A researcher is studying the regulation of the lac operon in E. coli and performing a northern blot analysis. They isolate mRNA from two bacterial strains: one with a wild-type lac operon genotype and another with a deletion mutation in the lacZ gene. The researcher hybridizes the mRNA with a probe specific to the lacZ gene.
What is the expected result of the northern blot analysis for the wild-type strain in a medium containing only glucose?
A researcher is studying the regulation of the lac operon in E. coli and performing a northern blot analysis. They isolate mRNA from two bacterial strains: one with a wild-type lac operon genotype and another with a deletion mutation in the lacZ gene. The researcher hybridizes the mRNA with a probe specific to the lacZ gene.
What is the expected result of the northern blot analysis for the wild-type strain in a medium containing only glucose?