12. Gene Regulation in Prokaryotes
Lac Operon
12. Gene Regulation in Prokaryotes Lac Operon
4PRACTICE PROBLEM
Suppose a microbiologist has created three partial diploid strains of E. coli, each with different genotypes for the lac operon genes. The researcher wants to determine whether the synthesis of lacZ mRNA is constitutive or inducible in each strain and whether the strain is able to utilize lactose as a carbon source.
Strain 1: cap⁻ I⁺ P⁺ O⁺ Z⁺/ cap⁺ I⁺ P⁺ O⁺ Z⁺
Strain 2: cap⁺ I⁺ P⁺ O⁺ Z⁺/ cap⁺ I⁺ P⁻ O⁺ Z⁺
Strain 3: cap⁺ I⁺ P⁺ O⁺ Z⁻/ cap⁺ I⁺ P⁺ O⁺ Z⁺
Which of the above strain is inducible in the presence of lactose?
Suppose a microbiologist has created three partial diploid strains of E. coli, each with different genotypes for the lac operon genes. The researcher wants to determine whether the synthesis of lacZ mRNA is constitutive or inducible in each strain and whether the strain is able to utilize lactose as a carbon source.
Strain 1: cap⁻ I⁺ P⁺ O⁺ Z⁺/ cap⁺ I⁺ P⁺ O⁺ Z⁺
Strain 2: cap⁺ I⁺ P⁺ O⁺ Z⁺/ cap⁺ I⁺ P⁻ O⁺ Z⁺
Strain 3: cap⁺ I⁺ P⁺ O⁺ Z⁻/ cap⁺ I⁺ P⁺ O⁺ Z⁺
Which of the above strain is inducible in the presence of lactose?