12. Gene Regulation in Prokaryotes
Lac Operon
12. Gene Regulation in Prokaryotes Lac Operon
7PRACTICE PROBLEM
A researcher wants to identify the lac operon gene that is mutated in a lac mutant strain of E. coli that does not produce β-galactosidase. To do this, they perform a complementation assay by transforming the lac mutant strain with a plasmid with the genotype lacI- lacZ+ to create a partial diploid strain.
Which of the following statements accurately describes the expected results of this complementation assay?
A researcher wants to identify the lac operon gene that is mutated in a lac mutant strain of E. coli that does not produce β-galactosidase. To do this, they perform a complementation assay by transforming the lac mutant strain with a plasmid with the genotype lacI- lacZ+ to create a partial diploid strain.
Which of the following statements accurately describes the expected results of this complementation assay?