Which of the following is true of cDNA produced using human brain...

Question

Which of the following is true of cDNA produced using human brain tissue as the starting material? 
(A) The procedure to make it requires amplification by the polymerase chain reaction. 
(B) It is produced from pre-mRNA using reverse transcriptase. 
(C) It can be labeled and used as a probe to detect genes expressed in the brain. 
(D) It includes the introns of the pre-mRNA.

Accepted Answer

Hi everyone. Let's look at our next question which of the following statements about C. D. N. A. Is false. So let's just think back to our content video about what C. D. N. A. What C. D. N. A. Is remembering that we are looking for the false or incorrect statement while C. D. N. A. Is A D. N. A copy from an M. RNA template. So instead of our natural process of having our D. N. A coding for a strand of M. R. N. A. The DNA being the template we're going the opposite direction. And one key thing to remember is that an M. R. N. A. The enTR ons have been excised. So when we look at our C. DNA molecule it doesn't contain the non coding regions of that original DNA genome. So just key difference to remember when you're looking at a sea DNA molecule and why do we want to do this? Um You can clone the C. D. N. A. Gene into a pro carry ah tick genome to produce the protein that it codes for in a cell that wouldn't normally produce it. So you can take the C. D. N. A. Gene, insert it into a bacteria and turn your bacteria into a little factory turning out the protein you're interested in. So let's look at remembering those things. Let's look at our answering answer choices, remembering that we are looking for a false statement choice A. Says synthesized from M. R. N. A. To reverse transcriptase. This is true. Um Reverse transcriptase. Easy to remember since we're going to reverse process RNA to DNA. So that's a true statement and therefore not our answer. Choice B says C. D. N. A. Is often used in gene cloning. Well that is true. Also as we discussed, you can use it to insert genes into pro chorionic cells and make a protein. So Choice B. Not our answer. Since it's a true statement, Choice E says it contains both coding and non coding sequences. Well this is false as we saw C. D. N. A. Doesn't contain the non coding regions since the introns were excised um and do not exist in our M. RNA template. So Choice C is a false statement and therefore must be our answer. And just to be thorough we'll look at choice D. Choice D says it is a double stranded DNA version of the M. RNA molecule. This is true. So Choice D. Not correct. So again, which the following statements about C. D. N. A false Choice C. Contains both coding and non coding sequences. Thanks for watching, we'll see you in the next video

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