Which of the following statement is incorrect regarding enhancers?

An enhancer is a specific region of DNA that can enhance the transcription of genes by interacting with transcription factors or modifying chromatin structure.

Enhancers can be located thousands of base pairs away from the gene they regulate.

Mutation in enhancers usually doesn't cause any significant change in the gene expression.

Enhancers can be located upstream, downstream, or even within an intron or another nearby gene.