15. Genomes and Genomics

Repetitive DNA poses problems for genome sequencing. Why is this so?

Restriction enzymes are proteins responsible for what?

What is the name of a short sequenced DNA fragment?

The purpose of a sequence assembly is to what?

Which of the following sequence techniques requires the use of vectors?

Dideoxy nucleotides (ddNTPs) are used in Sanger sequencing because they have what function?

You have discovered a new species of archaea from a hot spring in Yellowstone National Park. How would your strategy change if you were unable to grow the strain in culture?

How can we correlate the genome with RNA expression data in a tissue or a single cell?

How do we know which contigs are part of the same chromosome?

Repetitive DNA poses problems for genome sequencing. What strategies can be employed to overcome these problems?

Repetitive DNA poses problems for genome sequencing. What types of repetitive DNA are most problematic?

When the whole-genome shotgun sequence of the Drosophila genome was assembled, it comprised 134 scaffolds made up of 1636 contigs. How can physical gaps be closed?

When the whole-genome shotgun sequence of the Drosophila genome was assembled, it comprised 134 scaffolds made up of 1636 contigs. What is the difference between physical and sequence gaps?

When the whole-genome shotgun sequence of the Drosophila genome was assembled, it comprised 134 scaffolds made up of 1636 contigs. Why were there so many more contigs than scaffolds?