18. Biotechnology
Steps to DNA Cloning
Multiple Choice
Multiple ChoiceWhat is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments. V. Use ligase to seal plasmid DNA to non-plasmid DNA.
A
II, III, V, IV, I
B
III, II, IV, V, I
C
III, IV, V, I, II
D
IV, V, I, II, III
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