In a mutagenicity assay, S9 extract is added to Salmonella plates...

17. Mutation, Repair, and Recombination

Types of Mutations

17. Mutation, Repair, and Recombination

ANSWERS OPTIONS

Procarcinogens and mutagens are inert and cannot cause mutations unless they are activated by metabolic enzymes.

Salmonella bacteria are incapable of metabolizing procarcinogens and mutagens without the help of S9 extract.

The S9 extract provides a source of nutrients that enhances the growth of Salmonella bacteria, which in turn increases their mutagenicity..

S9 extract is a type of antibiotic that selectively kills Salmonella bacteria that have mutations.

In a mutagenicity assay, S9 extract is added to Salmonella plates to activate procarcinogens and mutagens. Which of the following best explains why this step is important in the assay?