18. Molecular Genetic Tools

In agarose gel electrophoresis used for DNA analysis, which DNA fragments move faster through the gel toward the positive electrode?

In methods for analyzing DNA, gel electrophoresis separates (sorts) DNA molecules primarily on the basis of their:

In agarose gel electrophoresis, what is the primary purpose of a DNA ladder?

In a standard DNA extraction protocol, what is the primary purpose of adding isopropyl alcohol to the lysate?

In forensic DNA analysis, which part of a human hair is most likely to contain sufficient nuclear DNA for STR profiling?

In a standard DNA extraction protocol, why is detergent (e.g., SDS) added to the sample?

In the context of methods for analyzing DNA and gene expression, what is a reporter gene?

In agarose gel electrophoresis, how are DNA or RNA molecules typically visualized on the gel after separation?

In DNA fingerprinting, which sections of DNA are most commonly analyzed to distinguish between individuals?

In a standard DNA extraction protocol, what is the primary purpose of adding detergent?

DNA fragments can be separated by gel electrophoresis because they differ in which property that affects their migration through the gel under an electric field?

In gel electrophoresis as a method for analyzing DNA, why is a DNA ladder (molecular weight marker) useful and why is it included on the gel?

DNA fingerprinting works because individuals (except identical twins) differ in the lengths and sequences of highly polymorphic regions of their DNA, which can be compared across samples. Which type of DNA variation is most commonly exploited in classic DNA fingerprinting?

In a standard DNA extraction protocol, what is the primary function of the lysis solution?

In agarose gel electrophoresis, how does DNA move through the gel when an electric field is applied?