10. Dynamics of Microbial Growth

Direct microscopic cell counting is a method where the microbiologist counts the number of microbes in a fraction of the microbial culture to:

Determining the number of cells within a culture is important. However, counting each and every cell with the human eye is tedious and extremely time consuming. Which of these is a method of cell counting that does not require a scientist to count each and every cell?

Match the term to the proper description(s). A term can have more than one description.

Choose the false statement about turbidity as an enumeration method.

a. It is an indirect enumeration method.

b. It is performed using a spectrophotometer.

c. It must be done using a liquid culture.

d. It differentiates between live and dead cells.

e. It is a rapid enumeration method.

A Coulter counter is a(n):

a. Statistical estimation using 15 dilution tubes and a table of numbers to estimate the number of bacteria per milliliter

b. Indirect method of counting microorganisms

c. Device that directly counts microbes as they pass through a tube in front of an electronic detector

d. Device that directly counts microbes that are differentially stained with fluorescent dyes

If there are 47 cells in 1 μl of sewage, how many cells are there in a liter?

List five direct methods of counting microbes.