9. Microscopes

Match the microscope with its function.

1. _____ Creates high contrast, 3D images of deep structures and time lapse images.
2. _____ Creates 2D images from a beam of electrons passing through a specimen.
3. _____ Creates images where the specimen's dense structures appear darker than the gray background.
4. _____ Allows the scientist to view stained or unstained specimens on a bright background.
5. _____ A light microscope with extremely high resolution.
6. _____ Creates 3D images from a beam of electrons scattering off a specimen's surface.
7. _____ Creates very detailed, high contrast, 3D images of live specimens.
8. _____ Allows the scientist to view specimens against a dark background.
9. _____ Creates high contrast, 3D images that show several planes of focus in the specimen.

Which type of microscope would be best to use to observe each of the following?

a. A stained bacterial smear

b. Unstained bacterial cells: the cells are small, and no detail is needed

c. Unstained live tissue when it is desirable to see some intracellular detail

d. A sample that emits light when illuminated with ultraviolet light

e. Intracellular detail of a cell that is 1μm long

f. Unstained live cells in which intracellular structures are shown in color

Three-dimensional images of live cells can be produced with:

a. Darkfield microscopy

b. Fluorescence microscopy

c. Transmission electron microscopy

d. Confocal microscopy

e. Phase-contrast microscopy

Which of the following is not a modification of a compound light microscope?

a. Brightfield microscopy

b. Darkfield microscopy

c. Electron microscopy

d. Phase-contrast microscopy

e. Fluorescence microscopy