Match the microscope with its function.

Question

List of microscope types for matching functions in microbiology course.

1. _____ Creates high contrast, 3D images of deep structures and time lapse images.
2. _____ Creates 2D images from a beam of electrons passing through a specimen.
3. _____ Creates images where the specimen's dense structures appear darker than the gray background.
4. _____ Allows the scientist to view stained or unstained specimens on a bright background.
5. _____ A light microscope with extremely high resolution.
6. _____ Creates 3D images from a beam of electrons scattering off a specimen's surface.
7. _____ Creates very detailed, high contrast, 3D images of live specimens.
8. _____ Allows the scientist to view specimens against a dark background.
9. _____ Creates high contrast, 3D images that show several planes of focus in the specimen.

Accepted Answer

Okay, everyone. Here we have a really giant practice problem, but a really good practice problem because it helps us review all the different types of microscopes that we've learned about. So I'm going to scroll down to attempt to allow us to see everything in one page. Okay. That looks good. We can see all the answer choices and all of the questions. So I'm gonna go through all of these different questions, and we're gonna try to match it to the microscope that we know has those characteristics. Number 1 says, creates high contrast 3 d images of deep structures and time lapse images. The keyword here for this particular type of microscope is time lapse. There's only one microscope that we've learned about that shows us time lapse images, which allows us to see how things move within a cell or within the body. And this is going to be the 2 photon microscope right here. So f is the first answer, and then we're going to cross that out. 2 creates 2 d images from a beam of electrons passing through a specimen. Keywords here are electron beam and 2 dimensional images. Remember that there's only 2 types of electron microscopes that we've talked about. 1 creates 3 d images, 1 creates 2 d images, and the little trick that I use to remember the difference is 2 starts with a t and the transmission electron microscope also starts with a t. So we're looking for t e m, so number 2's answer here is d. So this one right here, d. Okay. 3, creates images where the specimen's dense structures appear darker than the gray background. Okay. So most of the time in the microscopes that we've learned about, they have bright or dark backgrounds, but only one has a gray background and shows this types of microscopy shows dense structures, things like capsules around a cell or cell walls. And this is going to be phase contrast microscope. So 3, the answer is gonna be c. So then we're going to cross this one out, and then we should cross t, e, m, l before I forget. There we go. Okay. 4, this type of microscope allows the scientists to view stained or unstained specimens on a bright background, keyword here bright, because there's only one that has a stereotypically bright background and no other special characteristics, the bright field microscope. So 4 is gonna be b. So we're gonna cross that one out. 5, a light microscope with extremely high resolution. The one with extremely high resolution that is a light microscope is gonna be the newest technology in light microscopy. And this is going to be the super resolution microscope. It may its name tells you about its special characteristics. So 5 is going to be g. So we'll cross that out. Okay. 6, creates 3 d images from a beam of electrons scattering off a specimen's surface. So the keywords here are three-dimensional and electrons because there's only one other type of electron microscope in our answer choices and it does create 3 d images. That's going to be SEM or scanning electron microscope, which the answer here is h. Cross that out. Okay. 7, creates a very detailed, high contrast, 3 d images of live specimens. So the keywords here to recognize this microscope are three-dimensional and live specimens. There's actually only one microscope that we've talked about that doesn't actually have to kill the specimens to see them, and this is going to create three-dimensional images. A lot of the other microscopes do use techniques in their preparation that can damage or kill the organism that you're looking at, but this one definitely does not. This is going to be the differential interference contrast microscope. So I cross that guy out. 8, allows the scientists to view specimens against a dark background. So dark background seems to be the only important characteristic here, which means this must be the dark field microscope. So this answer is e. Cross that one out. We only have one answer left, but let's have a look at our question right here. Creates high contrast 3 d images that show several planes of focus in the specimen. This is going to be the confocal scanning laser microscope. Answer a. So we can cross that out. So here are all the different microscopes and their functions and their unique characteristics that you're gonna use to identify them. If you need any review on any of these microscopes, that's totally fine, there's a bunch of them, go back to our microscope review table. Okay, everyone. Let's move on to our next video.

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