18. Biotechnology

Gel Electrophoresis

Agarose Gel Electrophoresis

Agarose gel electrophoresis

Potato blight causes potato plants to shrivel and rot. The disease is caused by the pathogen Phytophthora infestans, infamous for its role in Ireland’s Great Potato Famine in the mid-1840s. The disease can devastate crops during wet weather, sometimes leading to total crop loss. Researchers aim to use recombinant DNA methods to transfer blight resistance genes from resistant varieties into susceptible varieties of potato.

Transgenic plants usually contain genes of bacterial plasmid origin. In a recent study, researchers designed a strategy that avoided using any plasmid genes. They transformed cells from a susceptible potato variety with a potato blight resistance gene cloned from a resistant variety. Next, to determine which plants from this group were also free of plasmid DNA (cloning vector) sequences, they performed PCR using primers specific for the plasmid. The positive control lane shows PCR amplification of plasmid DNA only, and the negative control lane shows an attempted PCR amplification of no added DNA. Based on the gel analysis of PCR products shown below, which plants contain only the potato gene? Explain your answer.

Molecular Biology

What is Gel Electrophoresis? | miniPCR bio™

 No. Host cells, such as bacteria, are often used to clone genes. 

 produce a large amount of DNA from a tiny amount of DNA 

 No. This is done by reverse transcriptase. 

identify genes that have been separated by electrophoresis or mRNA molecules through in situ hybridization

Consider the meaning of the word probe.

A nucleic acid probe is used to __________.

Gel Electrophoresis Example 1

Animation: Gel Electrophoresis of DNA

Gel electrophoresis | Chemical processes | MCAT | Khan Academy

DNA is negatively charged and attracted to the positive anode.

DNA is positively charged and attracted to the positive anode.

DNA is negatively charged and attracted to the positively charged agarose gel.

DNA is positively charged and attracted to the negatively charged agarose gel.

Why do the fragments of DNA in gel electrophoresis move away from the negative cathode?

Gel electrophoresis separates fragments of DNA based on which characteristic?

The blood at the crime scene does not belong to any of these suspects.

At a crime scene a blood sample was found and investigated using gel electrophoresis. Based on the gel, the blood at the crime scene belongs to which of the suspects?<img src="https://static.studychannel-prd.pearsonprd.tech/courses/biology/thumbnails/images/d2f5466d" alt="" />

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