What would be the consequence if eukaryotic cells did not undergo licensing during DNA replication?

• A. DNA replication would occur only once, ensuring accurate cell division.
• B. DNA replication would proceed normally without any impact on the cell cycle.
• C. DNA replication would occur multiple times in the same cell cycle, leading to genomic instability.
• D. DNA replication would be completely inhibited, preventing cell division.

Answer: (C)

Verified step by step guidance

1. Understand the concept of DNA replication licensing: Licensing is a regulatory mechanism that ensures DNA replication occurs only once per cell cycle in eukaryotic cells. It involves the assembly of pre-replicative complexes at replication origins during the G1 phase.
2. Recognize the role of licensing in preventing re-replication: Licensing prevents the re-initiation of DNA replication at the same origins within a single cell cycle, which is crucial for maintaining genomic stability.
3. Consider the consequences of missing licensing: Without licensing, replication origins could be activated multiple times within the same cell cycle, leading to multiple rounds of DNA replication.
4. Analyze the impact on genomic stability: Multiple rounds of replication without proper licensing can result in genomic instability, as the cell may end up with an abnormal number of DNA copies, potentially leading to mutations or cell malfunction.
5. Conclude the importance of licensing: Licensing is essential for ensuring that DNA replication is tightly regulated, preventing genomic instability and ensuring accurate cell division.