During what phase of mitosis do sister chromatids separate?

• A. Telophase
• B. Prophase
• C. Anaphase
• D. Metaphase

Answer: (C)

Verified step by step guidance

1. Understand the process of mitosis, which is the division of a single cell into two genetically identical daughter cells. Mitosis consists of several phases: prophase, metaphase, anaphase, and telophase.
2. Recall that sister chromatids are identical copies of a chromosome connected by a centromere. They are crucial for ensuring that each daughter cell receives an identical set of chromosomes.
3. Review the events of each mitotic phase: In prophase, chromosomes condense and become visible. In metaphase, chromosomes align at the cell's equatorial plane. In anaphase, sister chromatids are pulled apart toward opposite poles of the cell. In telophase, the cell prepares to divide, and nuclear membranes start to reform.
4. Focus on anaphase, where the key event is the separation of sister chromatids. This occurs when the centromeres split, allowing the chromatids to be pulled by spindle fibers toward opposite ends of the cell.
5. Conclude that the phase of mitosis during which sister chromatids separate is anaphase, as this is when they are physically divided and moved to opposite poles, ensuring each new cell will have a complete set of chromosomes.