Which of the following cell lysis techniques uses a high-frequency sound to disrupt the membrane?

• A. Sonication
• B. Mechanical homogenization
• C. Freeze-thaw cycles
• D. Detergent lysis

Answer: (A)

Verified step by step guidance

1. Understand the concept of cell lysis, which is the process of breaking down the cell membrane to release its contents.
2. Identify the different techniques used for cell lysis: mechanical homogenization, freeze-thaw cycles, detergent lysis, and sonication.
3. Mechanical homogenization involves physically breaking cells using shear forces, often with a blender or homogenizer.
4. Freeze-thaw cycles involve repeatedly freezing and thawing cells, causing ice crystals to form and disrupt the cell membrane.
5. Sonication uses high-frequency sound waves to create vibrations that disrupt cell membranes, effectively lysing the cells.