18. Biotechnology

Introduction to DNA Sequencing

How to sequence the human genome - Mark J. Kiel

What is Genomic Sequencing?

Researchers working on the Human Genome Project have determined the nucleotide sequences of human genes and in many cases identified the proteins encoded by the genes. Knowledge of the nucleotide sequences of genes might be used to develop lifesaving medicines or treatments for genetic defects. In the United States, both government agencies and biotechnology companies have applied for patents on their discoveries of genes. In Britain, the courts have ruled that a naturally occurring gene cannot be patented. Do you think individuals and companies should be able to patent genes and gene products?

HHMI BioInteractive: Sanger Method of DNA Sequencing

DNA sequencing | Biomolecules | MCAT | Khan Academy

Molecular Biology

DNA Sequencing - 3D

can be used to sequence entire eukaryotic chromosomes in a single reaction

No. A single reaction can sequence fragments up to about 800 nucleotides in length.

is very slow, requiring several weeks to determine a sequence of about 200 nucleotides

No. It is very fast; a typical reaction can read up to 800 nucleotides in a couple of hours.

No. The method produces many DNA fragments of slightly varying length, which are then separated by electrophoresis.

is difficult to automate and must be performed under close human supervision

No. The massive automation of DNA sequencing was a key element in the success of the Human Genome Project.

produces a set of labeled DNA fragments, each one ending at a different nucleotide

 This is the most common method for DNA sequencing.  <span data-etext-link="Concept 20.1"></span> 

The dideoxyribonucleotide method __________.  <span data-etext-link="Concept 20.1"></span>

Dideoxy Nucleotides

Introduction to DNA sequencing

Learn the toughest concepts covered in Biology1&2 with step-by-step video tutorials and practice problems by world-class tutors 

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1. Introduction to Biology

2. Chemistry

3. Water

4. Biomolecules

5. Cell Components

6. The Membrane

7. Energy and Metabolism

8. Respiration

9. Photosynthesis

10. Cell Signaling

11. Cell Division

12. Meiosis

13. Mendelian Genetics

14. DNA Synthesis

15. Gene Expression

16. Regulation of Expression

17. Viruses

19. Genomics

20. Development

21. Evolution by Natural Selection

22. Evolution of Populations

23. Speciation

24. History of Life on Earth

25. Phylogeny

26. Prokaryotes

27. Protists

28. Plants

29. Fungi

30. Overview of Animals

31. Invertebrates

32. Vertebrates

33. Plant Anatomy

34. Vascular Plant Transport

35. Soil

36. Plant Reproduction

37. Plant Sensation and Response

38. Animal Form and Function

39. Digestive System

40. Circulatory System

41. Immune System

42. Osmoregulation and Excretion

43. Endocrine System

44. Animal Reproduction

45. Nervous System

46. Sensory Systems

47. Muscle Systems

48. Ecology

49. Animal Behavior

50. Population Ecology

51. Community Ecology

52. Ecosystems

53. Conservation Biology

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Dideoxy Sequencing

The Steps of PCR

Introduction to Polymerase Chain Reaction

Steps to DNA Cloning

Gel Electrophoresis

DNA Fingerprinting

Southern Blotting

Introduction to DNA-Based Technology

Introduction to DNA Cloning

General Chemistry

Jules

Study the key concepts of Physics with descriptive videos, questions, and more

Physics

Patrick

Financial Accounting

Brian

Dive deep into Psychology with videos, practice problems, and more.

Psychology

Microeconomics

The first in a two-course sequence introduces the basic structure and functions of the human body

Anatomy & Physiology

College Algebra covers algebraic topics and skills necessary to succeed in college-level math

College Algebra

Organic Chemistry

Johnny

Genetics

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GOB Chemistry

Microbiology

Cell Biology

Analytical Chemistry

Study topics incliding trogonometric functions, identities and equations, solutions of triangles, complex numbers, and other topics as time permits

Trigonometry

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Precalculus

Biochemistry

Macroeconomics

Chemistry

Biology

Math

Business

Social Sciences

Michelle Barry Franco

Building Great Presentations

Eric Zackrison, PhD

Critical Thinking And Problem Solving

Sam Bennett

Embracing Times Of Uncertainty

Nicole Tetreault, PhD

Emotional Intelligence Basics

Eugene Choi, PharmD

Enhancing Your Critical Thinking Skills

Jason Richardson, PsyD

Habits To Win Every Day

Alyssa Lowery, PhD

Having Powerful Advanced Conversations

Don Gilman, EdD

How To Influence & Persuade

Amy Matthews

Leadership Skills For Rising Stars

Listening To Lead

Michael Shermer, PhD

Making Decisions Under Uncertainty

Lynne Hurdle

Mistakes To Avoid When Resolving Conflict

Michael Veltri

Overcoming Decision Making Traps

Mike Vardy

Prioritizing Effectively

Secrets Of Effective Prioritization

Strategies To Build Up Your Creativity

Tools For Decision Making

Tutti Taygerly

Unleash And Advance Your Creativity

Winning Collaboration: How To Work More Effectively With Different Personalities

Writing Emails People Want To Read

Critical Thinking

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Digital Literacy

Collaboration

Communication

Analytical Thinking

Leadership

Flexibility/Adaptability

Innovation

Eric Zackrison

Nicole Tetreault

Dive deep into Python with videos and more.

Introduction to Python

Arianne Dee

Agile Project and Product Management

Agile & Product Management

Mariya Breyther 

Introduction to Microsoft Power BI

Microsoft Power BI

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Data Analysis Fundamentals with Excel

Data Analysis - Excel

Chris Sorensen & Ammul Shergill

Getting Started with  and Cryptocurrency

Introduction to Blockchain

George Levy

Learn Enough HTML, CSS and Layout to be Dangerous

HTML, CSS & Layout

Lee Donahoe & Michael Hartl

Learn Enough JavaScript to be Dangerous: A Tutorial Introduction to Programming with JavaScript