What would be the best technique to prepare and view the internal structures of fungal hyphae?

• A. Scanning electron microscopy
• B. Transmission electron microscopy
• C. Phase-contrast microscopy
• D. Light microscopy with staining

Answer: (B)

Verified step by step guidance

1. Understand the purpose of each microscopy technique: Scanning electron microscopy (SEM) is used for detailed surface images, Transmission electron microscopy (TEM) is used for viewing internal structures, Phase-contrast microscopy enhances contrast in unstained samples, and Light microscopy with staining is used for general viewing with enhanced contrast.
2. Identify the goal of the observation: Since the problem asks for viewing internal structures of fungal hyphae, the technique should allow for detailed internal visualization.
3. Consider the capabilities of Transmission Electron Microscopy (TEM): TEM provides high-resolution images of internal structures by transmitting electrons through the sample, making it suitable for observing the internal details of fungal hyphae.
4. Evaluate the limitations of other techniques: SEM focuses on surface details, Phase-contrast microscopy is useful for live cells without staining but not for detailed internal structures, and Light microscopy with staining provides less resolution compared to TEM.
5. Conclude that Transmission Electron Microscopy (TEM) is the best technique for preparing and viewing the internal structures of fungal hyphae due to its ability to provide detailed images of internal cellular components.