During which stage of mitosis do chromosomes separate?

• A. Anaphase
• B. Metaphase
• C. Prophase
• D. Telophase

Answer: (A)

Verified step by step guidance

1. Understand the stages of mitosis: Mitosis is divided into several stages: prophase, metaphase, anaphase, and telophase.
2. Recall the key events in each stage: In prophase, chromosomes condense and become visible. In metaphase, chromosomes align at the cell's equatorial plate. In anaphase, sister chromatids separate and move toward opposite poles. In telophase, the cell prepares to divide as the nuclear envelope re-forms around each set of chromosomes.
3. Focus on the stage where separation occurs: The separation of sister chromatids is a defining event of anaphase.
4. Visualize the process: During anaphase, the spindle fibers shorten, pulling the sister chromatids apart toward opposite ends of the cell.
5. Conclude with the correct stage: Based on the understanding of mitosis stages, the correct answer is that chromosomes separate during anaphase.