Which of the following correctly places the steps involved in creating genetically modified organisms in their proper order?

• A. Transformation, isolation of gene, selection, insertion into vector
• B. Insertion into vector, selection, isolation of gene, transformation
• C. Isolation of gene, insertion into vector, transformation, selection
• D. Selection, transformation, insertion into vector, isolation of gene

Answer: (C)

Verified step by step guidance

1. Begin by isolating the gene of interest. This involves identifying and extracting the specific DNA sequence that you want to introduce into another organism.
2. Next, insert the isolated gene into a vector. A vector is a DNA molecule used as a vehicle to transfer the gene into the host organism. Common vectors include plasmids and viruses.
3. Proceed with the transformation step, where the vector containing the gene is introduced into the host organism's cells. This can be done using various methods such as electroporation or using a gene gun.
4. Finally, perform selection to identify and cultivate the organisms that have successfully incorporated the gene. This often involves growing the organisms in a medium that selects for those that have the vector, such as using antibiotic resistance markers.