18. Biotechnology

Dideoxy Sequencing

The Sanger Method of DNA Sequencing

How does Sanger Sequencing Work? – Seq It Out #1

Which of the following is NOT required for the reactions in dideoxy sequencing?

The dideoxy nucleotide prevents further synthesis of DNA due to the lack of a free 5’ carbon.

The dideoxy nucleotide prevents further synthesis of DNA due to the lack of a free 3’ OH.

The dideoxy nucleotide prevents further synthesis of DNA due to the lack of a nitrogen-containing base.

Chain termination is the same as sequencing by synthesis.

Dideoxy sequencing is also known as chain termination sequencing because:

Sanger Sequencing/Chain Termination Method

Chain-Termination PCR

How Sanger Sequencing Works? (Classic Sanger Method)

None of the above are the correct sequence.

According to the gel below, which of the following is the correct sequence on the unknown DNA molecule?<img src="https://static.studychannel.pearsonprd.tech/courses/biology/thumbnails/images/5f9ba8c0" alt="" />

It adds ddNTP to the end of each DNA fragment.

It changes the length of the DNA fragments.

It separates DNA fragments based on their sequence.

It separates DNA fragments generated during the sequencing reaction based on one nucleotide differences in their size.

The final step in a Sanger DNA sequencing reaction is to run the DNA fragments on a gel. What purpose does this serve?

Determining the DNA Sequence from a Gel

Components of Dideoxy Sequencing

Sanger Sequencing Method (Chain Termination DNA sequencing) Explained

How Does DNA Sequencing Work?

DNA sequencing | Biomolecules | MCAT | Khan Academy

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