By analyzing the Southern Blot results below, which of the samples contains the gene of interest?

• A. A only.
• B. A & C.
• C. B & D.
• D. A, C, & E.
• E. E only.
• F. None.

Answer: (D)

Verified step by step guidance

1. Understand the Southern Blot technique: It involves transferring DNA fragments from a gel to a filter paper and then probing with a labeled DNA probe to detect specific sequences.
2. Examine the gel image on the left: It shows DNA fragments separated by size, with each lane labeled A to E. The bands represent DNA fragments of different sizes.
3. Look at the filter paper image on the right: This shows the results after hybridization with the labeled probe. Only the bands that hybridize with the probe will be visible.
4. Identify the lanes with visible bands on the filter paper: These indicate the presence of the gene of interest. In this case, lanes A, C, and E have visible bands.
5. Conclude which samples contain the gene of interest: Based on the visible bands on the filter paper, samples A, C, and E contain the gene of interest.