How many NADH molecules are produced during glycolysis?

• A. 2 NADH
• B. 1 NADH
• C. 3 NADH
• D. 4 NADH

Answer: (A)

Verified step by step guidance

1. Understand the process of glycolysis: Glycolysis is the metabolic pathway that converts glucose into pyruvate, releasing energy and producing ATP and NADH in the process.
2. Identify the key steps in glycolysis where NADH is produced: During glycolysis, NADH is generated in the sixth step, where glyceraldehyde-3-phosphate is converted to 1,3-bisphosphoglycerate.
3. Recognize the role of NAD+ in glycolysis: NAD+ acts as an electron carrier, accepting electrons during the oxidation of glyceraldehyde-3-phosphate, which results in the formation of NADH.
4. Calculate the number of NADH molecules produced: Since glycolysis involves the conversion of one molecule of glucose into two molecules of pyruvate, and each conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate produces one NADH, two NADH molecules are produced per glucose molecule.
5. Review the options provided: Compare the calculated number of NADH molecules produced during glycolysis with the given options to identify the correct answer.