18. Biotechnology

Southern Blotting

Difference between Southern and northern blotting and western blotting

Southern Blot

Southern blot | Biomolecules | MCAT | Khan Academy

Southern Blot Method - Animation video

A _____ is a single-stranded DNA molecule used in hybridization reactions to detect the presence of a particular gene in an assortment of DNA fragments. (Hybridization Reactions: reactions that combine two complementary single-stranded DNA molecules)

By analyzing the Southern Blot results below, which of the samples contains the gene of interest?<img src="https://static.studychannel.pearsonprd.tech/courses/biology/thumbnails/82fb08f4" alt="" />

A radioactive probe, which is complementary to Gene X, will bind to the membrane.

A radioactive probe, which has the same sequence as Gene X, will bind to the membrane.

A radioactive probe will be absent from the membrane.

A southern blot cannot confirm if the patient possesses Gene X.

A geneticist wants to see if her patient has Gene X. The geneticist takes a blood sample from her patient and prepares a southern blot. How will the geneticist know if her patient possesses Gene X?

Steps of Southern Blotting

The Principle of Southern Blotting and Northern Blotting, Blotting Techniques, The Full Mechanism

Place the following steps of Southern Blotting in the correct order. a) ______: Filter paper is incubated with the labeled DNA probe which anneals to the ssDNA fragments. b) ______: Analyze gel to determine the presence of the DNA sequence of interest. c) ______: Separate DNA fragments by size using gel electrophoresis. d) ______: Denature DNA by soaking gel in a basic solution. e) ______: Fragment unknown DNA sample(s) using restriction enzymes.

A southern blotting technique is used to detect a specific ______ sequence from a blood or tissue sample.

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