Disruption of the active site of an enzyme would most likely result in which of the following?

• A. Enhanced enzyme specificity
• B. Loss of enzyme activity
• C. Increased substrate concentration
• D. Formation of more enzyme-substrate complexes

Answer: (B)

Verified step by step guidance

1. Understand the role of the active site: The active site of an enzyme is the region where substrate molecules bind and undergo a chemical reaction. It is specifically shaped to fit the substrate, facilitating the conversion of substrate to product.
2. Consider the effect of disruption: Disruption of the active site can occur due to changes in pH, temperature, or the presence of inhibitors. Such changes can alter the shape or charge properties of the active site, preventing the substrate from binding effectively.
3. Analyze the impact on enzyme activity: If the active site is disrupted, the enzyme may no longer be able to bind to the substrate, leading to a decrease or complete loss of enzyme activity. This is because the enzyme-substrate complex cannot form, which is essential for the catalytic process.
4. Evaluate the options: Enhanced enzyme specificity would require a more precise fit between the enzyme and substrate, which is unlikely if the active site is disrupted. Increased substrate concentration does not compensate for the inability of the enzyme to bind the substrate. Formation of more enzyme-substrate complexes is also unlikely if the active site is compromised.
5. Conclude with the most likely outcome: Given the disruption of the active site, the most probable result is a loss of enzyme activity, as the enzyme can no longer effectively catalyze the reaction.