Which enzyme is responsible for splitting the two strands of DNA during replication?

• A. Ligase
• B. Helicase
• C. Polymerase
• D. Topoisomerase

Answer: (B)

Verified step by step guidance

1. Understand the process of DNA replication: DNA replication is the process by which a cell duplicates its DNA, resulting in two identical copies. This process is crucial for cell division.
2. Identify the role of enzymes in DNA replication: Several enzymes are involved in DNA replication, each with a specific function. These include helicase, polymerase, ligase, and topoisomerase.
3. Focus on the function of helicase: Helicase is the enzyme responsible for unwinding the DNA double helix. It breaks the hydrogen bonds between the two strands, allowing them to separate and serve as templates for replication.
4. Differentiate between the enzymes: Ligase is involved in joining DNA fragments, polymerase synthesizes new DNA strands, and topoisomerase helps relieve the tension in the DNA strand during replication.
5. Conclude with the specific role of helicase: Helicase is the enzyme that initiates the replication process by splitting the two strands of DNA, making it possible for other enzymes to synthesize new strands.