At what point do sister chromatids separate?

• A. G₁ phase
• B. G₂ phase
• C. Metaphase
• D. prophase
• E. Anaphase

Answer: (E)

Verified step by step guidance

1. Understand the cell cycle phases: The cell cycle consists of interphase (G1, S, G2 phases) and the mitotic phase (M phase), which includes prophase, metaphase, anaphase, and telophase.
2. Identify the role of sister chromatids: Sister chromatids are identical copies of a single chromosome that are connected by a centromere. They are crucial for accurate genetic material distribution during cell division.
3. Review the events of mitosis: During mitosis, the sister chromatids must be separated to ensure each daughter cell receives an identical set of chromosomes.
4. Focus on anaphase: In anaphase, the centromeres split, and the spindle fibers pull the sister chromatids apart toward opposite poles of the cell. This separation is a key event in mitosis.
5. Conclude that sister chromatids separate during anaphase, which is the phase following metaphase where the chromatids align at the cell's equatorial plane.